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Anesthesia in Pacific oyster, Crassostrea gigas ArchiMer
Suquet, Marc; De Kermoysan, Goulwen; Araya, Ricardo Gonzalez; Queau, Isabelle; Lebrun, Luc; Le Souchu, Pierrick; Mingant, Christian.
A reliable anaesthesia and sampling protocol for Pacific oysters will enable experiments to be conducted without sacrificing animals and will facilitate successive sampling of individuals for gametogenesis studies. As no such techniques were available for Crassostrea gigas, the present study aimed to define suitable anaesthetic conditions for use with this species. Three groups of ten oysters (mean weight +/- SD, 32.1 +/- 9.0 g) were anaesthetised in 5 L containers. Among different chemicals: benzocaine, eugenol and three different types of magnesium chloride (a laboratory one Flucka (R) - and two designed for agriculture - DEUSA International (R) and Dead SeaWorks (R)) and concentrations tested, one laboratory (concentration: 72 g L-1) and one...
Tipo: Text Palavras-chave: Crassostrea gigas; Pacific oyster; Gonad sampling; Magnesium chloride; Relaxant; Anaesthesia.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6352.pdf
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Effect of phytoplankton and temperature on the reproduction of the Pacific oyster Crassostrea gigas: investigation through DEB theory ArchiMer
Bernard, Ismael; De Kermoysan, Goulwen; Pouvreau, Stephane.
DEB theory can be used to obtain a detailed description of energy allocation in organisms and the control of this allocation by temperature and food concentration. In this study, we modified the model of Bourlès et al. (2009) developed for the Pacific oyster, Crassostrea gigas, to improve the description of reproductive effort. The model was amended in two ways: a new set of parameters was incorporated and a full description of gonad construction in spring was added, with a new state variable. The application of this model to a dataset for oyster growth measured in four bays over two years showed that this model better described reproductive effort, without modifying overall growth dynamics. We then explored the reproductive responses of oysters to their...
Tipo: Text Palavras-chave: DEB model; Crassostrea gigas; Gametogenesis; Reproductive effort; Spawning date; Functional response.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00059/17047/14606.pdf
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